Southwest Animal Imaging Resource, University of Arizona

SWAIR Home: BMR Capabilities

Biological Magnetic Resonance Capabilities

Anatomic Imaging: High resolution MRI of mice, rats, rabbits and other small animals - Specialized imaging protocols developed for imaging specific anatomy (e.g. brain, breast, liver)

Functional and Physiologic Imaging: New protocols and capabilities developed for specific applications - Existing capabilities for: Angiography (MRA) Dynamic Contract Enhancement (DCE) Diffusion and Perfusion Imaging pH imaging Magnetic Transfer Contract (MTC) Imaging functional MRI (fMRI)

Magnetic Resonance Spectroscopy: High resolution ¹D and ²D, ¹H, ¹³C, ³¹P, ¹⁹F NMR spectroscopy - Single voxel in vivo spectroscopy - Chemical-shift imaging (CSI)

In Vivo Bioluminescence: This technology allows the non-invasive imaging and quantification of cells expressing luciferase proteins. The major luciferase used in these studies is from the firefly, phytonis pyralis.

Specialized experience with: Bioreactor system - Tumor Models - Transgenic animals - MRI compatible physiologic monitor

Protein Structure & Dynamics NMR:

For details about available equipment visit: bmr.arl.arizona.edu

Faculty

Robert J. Gillies, Ph.D.
PI - SWAIR Resource
Professor of, Radiology, Biochemistry & Molecular Biophysics, Physiology
Director of Advanced Research Institute in Biomedical Imaging (ARIBI)
Director, Cancer Imaging and Technology Program, Arizona Cancer Center
University of Arizona
Arizona Cancer Center
1515 N. Campbell Avenue
Tucson, Arizona 85724
(520) 626-5050
gillies@email.arizona.edu

Art Gmitro, Ph.D.
PI - MR Component
Professor of Radiology and Optical Sciences
University of Arizona
Department of Radiology
AHSC 1564 PO Box 245067
Tucson, Arizona 85724
(520) 626-4720
gmitro@radiology.arizona.edu www.optics.arizona.edu/faculty/resumes/gmitro.htm

Constantin Job
Arizona Research Laboratories
University of Arizona
P.O. Box 210077
Tucson, AZ 85721
(520) 621-8521
cjob@email.Arizona.EDU

Jean-Philippe Galons, Ph.D.
Research Assistant Professor
University of Arizona
Department of Radiology
PO Box 245067
Tucson, Arizona 85724
(520) 626-3337
jgalons@email.arizona.edu
http:// www.radiology.arizona.edu/~research/galons.htm

Theodore Trouard, Ph.D.
Associate Professor
University of Arizona
Biomedical Engineering AHSC 1564
PO Box 245067
Tucson, Arizona 85724
(520) 626-2177
trouard@radiology.arizona.edu http://www.radiology.arizona.edu/~research/trouard.htm

Maria Altbach, Ph.D.
Assistant Professor
University of Arizona
Department of Radiology AHSC 1564
PO Box 245067
Tucson, Arizona 85724
(520) 626-5532
maltbach@email.arizona.edu
http:// www.radiology.arizona.edu/~research/altbach.htm

Natarajan Raghunanad, Ph.D.
Research Associate Professor, Radiology
Director, Translational Cancer Imaging
Arizona Cancer Center
raghunan@email.Arizona.EDU
(520) 626-6041/626-5050
www.biochem.arizona.edu/raghunand

Denzheng Zhao, Ph.D.
Research Associate, ARL Division
(520) 626-3337
dzhao@email.arizona.edu

Gary Martinez, Ph.D.
Research Assistant Professor
Arizona Cancer Center
(520) 626-3337

Staff and Graduate Students

Staff

Merry Warner, B.A.
Senior Program Coordinator
warnerm@email.arizona.edu
520-626-5050
520-626-5051 (fax)

Christine Howison, B.S.
Animal Technician
BMR Facility
chowison@email.Arizona.EDU
520-626-6653
520-821-8522 (fax)

Matt Runquist, B.S.
Projects Coordinator
BMR Facility
runquism@email.Arizona.EDU
520-626-6653
520-821-8522 (fax)

Brenda Baggett, B.S.
Research Specialist
Arizona Cancer Center
baggett@u.arizona.edu
520-626-5052
520-626-5051 (fax)

Grad Students

Dominique Jennings, B.S.
BME

Heather Woodin, B.S.
MCB

Robert Kaufman, B.S.
MCB

Xiaomeng Zhang, B.S.
BME

Ren Stephen, B.S.
Nutrition

Research Projects

Genetic and molecular analyses of mutations in collaboration with the Dr. Brilliant (Department of Pediatrics, UofA). The project consists in observing in situ the palate formation in embryos presenting a single genetic mutation. Current status: A number of technical challenges specific to the manipulation of pregnant mice have been resolved, a protocol has been defined.

Detection of Chemotherapeutic response by Diffusion-MRI in collaboration with Dr. Powis, (Arizona Cancer Center). Several sub-projects using diffusion weighting are currently under way. Current status: The protocols and methods for this work have been defined.

Antiangiogenic therapy of prostate cancer in collaboration with Dr. Oleson (Department of Oncology, UofA). Current status: Preliminary studies have been performed. A data analysis method for dynamic contrast imaging is being developed.

Study of vascular fraction during ischemic revascularization in collaboration with Dr. Hoying, (Bioengineering, UofA). Current status: The protocol has been defined. Preliminary data acquisition studies are underway.

Relevant Publications

Raghunand N, Gillies RJ (2001) Nuclear Magnetic Resonance (NMR) Spectroscopy in the Study of Whole-Body Metabolism. Encyclopedia of Life Sciences 1-6.

Gillies RJ (2001) The Tumour Microenvironment: Causes and Consequences of Hypoxia and Acidity. Novartis Foundation Symposium 240. John Wiley & Sons, Ltd., England.

Gillies RJ, Raghunand N, Garcia-Martin ML, Gatenby RA (2004) pH Imaging. A Review of pH Measurement Methods and Applications in Cancers. IEEE Engin Med Biol Magazine Sept/Oct:57-64.

Raghunand N, Gatenby RA, Gillies RJ (2003) Microenvironmental and cellular consequences of altered blood flow in tumors. Br J Radiology 76 Suppl 1:S11-22.

Vagner J, Handl HL, Gillies RJ, Hruby VJ (2004) Novel targeting strategy based on multimeric ligands for drug delivery and molecular imaging: homooligomers of alpha-MSH. Bioorg Med Chem Lett. 14(1):211-5.

Handl HL, Vagner J, Yamamura HI, Hruby VJ, Gillies RJ (2004) Lanthanide-based time-resolved fluorescence of in cyto ligand-receptor interactions. Analytical Biochemistry 330:242-250.

Theilmann RJ, Borders R, Trouard TP, Xia G, Outwater E, Ranger-Moore J, Gillies RJ, Stopeck A (2004) Changes in water mobility measured by diffusion MRI predict response of metastatic breast cancer to chemotherapy. Neoplasia 6(6):831-837.

Baggett B, Roy R, Momen S, Morse D and Gillies RJ (2004) Thermostable luciferase mutants improve the sensitivity as reporters for in vivo bioluminescence. Molecular Imaging 3(4):324-332.

Morse DL and Gillies RJ (2004) Recent Advances in MR Imaging and Spectroscopy: Choline containing compounds as diagnostic, prognostic and therapeutic response indicators for breast cancer. N.R. Jagannathan, Ed. Jaypee Brothers Medical Publishers, New Delhi.

Gatenby RA and Gillies RJ (2004) Why do Cancers have High Aerobic Glycolysis? Nature Reviews 4:891-899.

Gillies RJ, Raghunand N, Garcia-Martin ML, Gatenby RA (2004) pH Imaging: A review of pH measurements methods and applications in Cancers. IEEE Engineering in Medicine and Biology Magazine 23(5):57-64.

Handl HL, Vagner J, Han H, Mash E, Hruby VJ, and Gillies RJ (2004) Hitting Multiple Targets with Multimeric Ligands. Expert Opinion on Targeted Therapy 8(6):565-586.

Jordan BF, Runquist M, Raghunand N, Gillies RJ, Tate WR, Powis G, Baker A (2005) The thioredoxin-1 inhibitor PX-12 decreases vascular permeability as monitored by dynamic contrast enhanced MRI, comparison with VEGF production in xenograft tumors. Clinical Cancer Research 11:529-536.

Morse D, Gray H, Payne C, Gillies RJ (2005) Taxotere induces cell death through mitotic catastrophe. Molec. Cancer Therapeutics 342:69-77.

Galons J-P, Altbach MI, Taylor CW, Payne-Murrietta G and Gillies RJ (1999) Early Increases in Breast Tumor Xenograft Water Mobility in Response to Paclitaxel Therapy Detected by Non-Invasive Diffusion Magnetic Resonance Imaging. Neoplasia 1(2):113-117.

Fregosi RF, Quan SF, Kaemingk KL, Morgan WJ, Goodwin JL, Cabrera R and Gmitro A (2003) Sleep-Disorded Breathing, Pharyngeal Size and Soft Tissue Anatomy in Children. J. Appl. Physiol. 95:2030-2038.

De Weerd P, Reinke K, Ryan L, McIsaac T, Perschler P, Schnyer D, Trouard T, and Gmitro A (2003) Cortical Mechanisms for Acquisition and Performance of Bimanual Motor Sequences. Neuroimage 19:1405-1416.

Theilmann RJ, Gmitro AF, Altbach MI, Trouard TP (2004) View Ordering Techniques in Radial Fast Spin-Echo Imaging. Magn. Reson. Med., 51, 768-774 (2004).

Rouse, A.R., Kano, A., Udovich, J.A., Kroto SM and Gmitro AF (2004) Design and Demonstration of a Miniature Catheter for a Confocal Microendoscope. Appl. Opt. 43:5763-5771.

Corum CA and Gmitro AF (2004) Spatially Varying Steady State Longitudinal Magnetization in Distant Dipolar Field-based Sequences. J. Magn. Reson. 171:131-134.

Brewer MA,Utzinger U, Barton JK, Hoying JB, Kirkpatrick ND, Brands WR, Davis JR, Hunt K, Stevens SJ, Gmitro AF (2004) Imaging of the Ovary. Technology in Cancer Research and Treatment, 3:617-627.

Gmitro AF, Kono M, Theilmann RJ, Altbach MI, Li Z, and Trouard TP (2005) Radial GRASE: Implementation and Applications. Magn. Reson. Med., in press.

Sarlls J, Newbould R, Altbach MI, Gmitro AF, Seeger J, Trouard TP (2005) Isotropic Diffusion Weighting in Radial Fast Spin Echo (Radial-FSE) MRI. Magn. Reson. Med. in press.

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